cell culture l6 skeletal muscle cells  (ATCC)

Journal: Turkish Journal of Pharmaceutical Sciences

Article Title: Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells

doi: 10.4274/tjps.08108

Figure Lengend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680), and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum rat L6 skeletal muscle myotubes (70-90% confluent) were serum starved for 7 days and then stimulated for 1 hour IL-6 mRNA levels were measured relative to TATA-BOX using real-time quantitative polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (10 μM), PSB 603 (100 nM and 1 μM), CGS21680 (100 nM), and forskolin (100 nM), data were represented as means ± standard error of mean of at least three independent experimental groups *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001, data were analysed using a one-way ANOVA test followed by a Tukey test

Article Snippet: Cell culture Rat L6 skeletal muscle cell line and myoblast cell line were originally obtained from the American Type Culture Collection (USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: Turkish Journal of Pharmaceutical Sciences

Article Title: Adenosine A2B Receptors - Mediated Induction of Interleukin-6 in Skeletal Muscle Cells

doi: 10.4274/tjps.08108

Figure Lengend Snippet: Effects of 5’-N-ethylcarboxamidoadenosine (NECA), 2-(4-[2-carboxyethyl]-phenethylamino) adenosine-52-nethyluronamide (CGS21680) and 8-[4-[4-(4-chlorophenzyl) piperazide-1-sulfonyl) phenyl]]-1-propylxanthine (PSB 603) on interleukin 6 (IL-6) messenger RNA (mRNA) gene expression in rat L6 skeletal muscle myotubes using charcoal serum, rat L6 skeletal muscle myotubes were stimulated for the indicated time from 1 hour to 24 hours and interleukin-6 mRNA levels, relative to TATA-BOX, was measured by quantitative real time polymerase chain reaction; stimulation was performed with vehicle (0.1% dimethyl sulphoxide), NECA (100 nM and 10 μM), PSB 603 (10 μM), CGS21680 (100 nM) and forskolin (1 μM) (a) Stimulation was performed up to 1 hour (b) Stimulation was performed for up to 3 hours (c) Stimulation was performed for up to 24 hours, data were represented as means ± standard error of mean of three independent experimental group (n=3; *denotes p<0.05, **denotes p<0.01 and ***denotes p<0.001), data were analyzed using one way ANOVA test followed by Tukey test

Article Snippet: Cell culture Rat L6 skeletal muscle cell line and myoblast cell line were originally obtained from the American Type Culture Collection (USA).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction

Journal: American Journal of Physiology - Cell Physiology

Article Title: Dexamethasone stimulates store-operated calcium entry and protein degradation in cultured L6 myotubes through a phospholipase A 2 -dependent mechanism

doi: 10.1152/ajpcell.00309.2009

Figure Lengend Snippet: Table 1.

Article Snippet: Cell Cultures Rat L6 skeletal muscle cells were purchased from the American Type Culture Collection (Manassas, VA).

Techniques: Cell Culture